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Mouse Syndecan 1 (SDC1;CD138) ELISA Kit

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Product Name:

Mouse Syndecan 1 (SDC1;CD138) ELISA Kit

Cat.#:

JL20195

Regulatory Status:
Alternative:

CD138; SDC; SYND1; Syndecan Proteoglycan 1

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
6.25-400ng/mL
Sensitivity:
3.98ng/mL
Specificity:
This kit recognizes Mouse SDC1;CD138 in samples. No significant cross-reactivity or interference between Mouse SDC1;CD138 and analogues was observed.
Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Procedure:
Assay Procedure
Research Area:
Cancer
  • Mouse Syndecan 1 (SDC1;CD138) ELISA Kit
  • Mouse Syndecan 1 (SDC1;CD138) ELISA Kit
  • Mouse Syndecan 1 (SDC1;CD138) ELISA Kit
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Product Datasheets
Instruction: Down Instruction


| SDC1;CD138

Syndecan 1 is a protein which is encoded by the SDC1 gene.The protein is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a sponge for growth factors and chemokines, with binding largely via heparan sulfate chains. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 
Concentration (ng/mL) 400 200 100 50 25 12.5 6.25 0
OD 2.32 1.5 0.87 0.52 0.35 0.24 0.18 0.08
Corrected OD 2.24 1.42 0.79 0.44 0.27 0.16 0.1 -
 





| Repeatability

Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%
 

| Recovery

The recovery of Mouse SDC1;CD138 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 
Sample Type Range(%) Average Recovery(%)
Serum(n=8) 86-101 96
Plasma(n=8) 92-106 102
Cell Culture Supernates(n=8) 96-108 103





 

| Linearity

Samples were spiked with high concentrations of Mouse SDC1;CD138 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 
    Serum(n=4) Plasma(n=4) Cell Culture Supernates(n=4)
1 : 2 Range (%) 84-95 88-96 90-110
Average (%) 90 93 96
1 : 4 Range (%) 89-103 87-108 105-115
Average (%) 94 98 108







 
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Sample Data
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Sample Data
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Sample Data
Size Quantity Price ($)
48T
1
360 / 360
96T
1
460 / 460
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